細(xì)胞衰老是細(xì)胞停止分裂的現(xiàn)象。衰老可由內(nèi)部(氧化應(yīng)激、DNA損傷等)和外部(毒素、紫外線等)因素誘導(dǎo)。衰老是一種生長(zhǎng)停滯的狀態(tài),細(xì)胞在這種狀態(tài)下保持活力但停止分裂。衰老細(xì)胞表現(xiàn)出細(xì)胞大小增加,β-半乳糖苷酶、p53和P16活性的衰老相關(guān)表達(dá),以及基因表達(dá)模式的改變。
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SenTraGor™ (GL13) is an innovative reagent for the detection of senescence. Senescent cells play a potent role in normal development, tissue homeostasis, aging and a wide spectrum of age related disorders. Up to now, a major problem in the field of senescence was the absence of a reliable marker to specifically detect these cells, especially in situ. SenTraGor™ (GL13) is a biotinylated Sudan Black B based chemical reagent that was designed to overcome this constraint.
SenTraGor™ (GL13) staining allows the detection of senescence in any biological material. Direct visualization of senescent cells can be achieved in all archival tissue specimens (formalin-fixed paraffin-embedded) and cells from biopsies or cell cultures.
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